Within the oral cavity, saliva shows great promise as a noninvasive and sensitive marker for many systemic diseases. Salivary analysis of oral cancer biomarkers british journal. In saliva, the tumorspecific dna was positive in 100% of the patients diagnosed with oral. Salivary protease spectrum biomarkers of oral cancer. Reliable biomarkers for oral cancer oc remain scarce, and routine tests for the detection of precancerous lesions are not routine in the clinical setting. A new approach for the diagnosis of systemic and oral. Salivary biomarkers represent a promising noninvasive approach for oral cancer detection, and an area of strong research interest. More accurate oral cancer screening with fewer salivary. Salivary biomarkers for detection of oral squamous cell carcinoma. With the development of salivaomics, increased numbers of biomarkers that are related to oral and systemic diseases have been identified in the saliva.
The aim of this study was to investigate if certain salivary biomarkers could be used for. Oral squamous cell carcinoma oscc is one of the most common deadly diseases with a poor survival rate of 60%. Many of these constituents, their altered levels in oral cancer can serve as the potential biomarker and aid in its diagnosis. Oral cancer is one of the most common cancers in the world, representing a serious problem for global health shah and gil, 2009. Considering previously published results, we can infer that these salivary biomarkers are useful for oral cancer detection regardless of ethnicity. We describe the distinct features of the tongue, mucosa, and salivary glands. Salivary biomarkers list of high impact articles ppts. An insight on salivary biomarkers for oral cancer abstract oral cancer is one of the major public health concerns with 12% of all types of cancers worldwide. Considering previously published results, we can infer that these salivary biomarkers are useful for oral cancer detection regardless of. We tested 19 tongue cancer patients, measuring the.
In saliva, the tumorspecific dna was positive in 100% of the patients diagnosed with oral cancer, and 4770% of the patients with tumors in other places of the body also carry specific tumor dna markers in the saliva. Salivary tools for the measurement and investigation of alterations in particular salivary molecules such as. Pdf oral cancer is one of the major global public health problems and is the sixth most common human malignancy. Lawrence,dds, msc, phd abstract because of interest in the link between oral. The discovery of these new targets may lead to a simple clinical tool for the noninvasive diagnosis of oral cancer. Pdf oral cancers are the sixth most frequent cancer with a high mortality rate. Direct contact between saliva and the oral cancer lesion makes measurement of tumour. Oral cancer is the sixth most common cancer type in the world, and 90% of it is represented by oral squamous cell carcinoma oscc. Clinical significance of salivary biomarkers in oral squamous cell. Despite the groundbreaking findings demonstrated, the authors assert that the ongoing strategy for detecting oscc oral visual inspection and biopsy is ineffective, because it has globally yielded high specificity but varied sensitivity. The 90% of oral cancer issues have been increased due to smoking and alcohol consumption all over the world. Oral squamous cell carcinoma oscc accounts for more than 90% of all oral cancer. Salivary nglycosylation as a biomarker of oral cancer. More than 100 potential salivary biomarkers have been reported till date.
Salivary biomarkers for oral squamous cell carcinoma. Numerous studies have described the potential of mirnas as cancer biomarkers for oral cancer 72, 73. In a recently published study, approximately 50 mirnas were detected in saliva using reverse transcriptasepreamplificationquantitative sensitivity %specificity %auc polymerase chain reaction. In addition, chronic periodontitis is a risk indicator for oral cavity andor oropharyngeal cancer 12. However, some issueschallenges have arisen that need to be resolved in order to establish this approach as a reliable, highly sensitive and specific method for clinical use. Introduction and application of biomarkers to oral cancer.
Usefulness of salivary biomarkers in oral precancer and cancer alberto rodriguezarchilla, m. Search for articles involved the medline, pubmed, and embase. Pdf salivary biomarkers for oral cancer and precancer screening. Such a choice was taken in order to exclude presence of pathologies in the proximity of the site. Roughly 90% of oral cancers histologically originate from squamous cells and are classified as oral squamous cell carcinoma oscc rivera, 2015. This has opened a new avenue for salivary biomarker discovery for human diseases. This study addresses a current unmet need for more sensitive and quantitative tools for the management of oc. As overviewed in table 3, salivabased microbial biomarkers have also been linked to oral cancer and even obesity. Our results support the idea that salivary diagnostics might be used in the clinical practice. Longterm longitudinal studies with large populations of individuals with oral. Nonetheless, emerging barriers are an obstacle to the study of the salivary proteome in an effective way. The combination of transcriptomic and proteo mic salivary markers is of great value for oral cancer detection and differentiation from pmod patients. We tested 19 tongue cancer patients, measuring the levels of 8 salivary markers related to oxidative stress. Salivary il8, il6 and tnf as potential diagnostic biomarkers for oral cancer haafsa arshad sahibzada 1, zohaib khurshid 2, rabia sannam khan 3, mustafa naseem 4, khalid mahmood siddique 5, maria.
Beatriz carrionruiz department of stomatology, faculty of dentistry, oral medicine unit, university of. Patients with oral cancer have elevated levels of certain protease enzymes in their saliva, a finding that could help with screening and diagnosis. Salivary biomarkers in psychological stress diagnosis. Role of salivary biomarkers in oral cancer detection. However, inquiries of this nature are not uncommon. Accepted papers reported at least 2 separate estimates. Numerous analytes have been detected in saliva using various techniques table 2. Toward future clinical and diagnostic utilities janice m. Initially, technical and methodologic information was transferred, via handson training, to the operator in an nciedrnsponsored laboratory. Over the past two decades, using salivary biomarkers specifically for early cancer detection has attracted much research interest, especially for cancers occurring in the oral cavity and oropharynx, for which the fiveyear survival rate 62% is still one of the lowest among all major human cancers. Role of salivary biomarkers in the diagnosis of oral cancer jmrps. Moreover, the unstable rna is protected in exosomes, allowing their stable detection and easy quantification.
Salivary biomarkers for oral cancer and precancer screening. We subjected our salivary oral cancer biomarkers to a phase ii validation study with an. Salivary biomarkers and their efficacies as diagnostic tools for oral squamous cell carcinoma. Several salivary protein markers such as interleukins 8, 6, 1. The nciedrn is a dedicated entity committed to make molecular diagnostics for cancer a clinical reality. Salivary biomarkers of stress can minimize some of the potential confounding variables associated with blood sampling 3.
Usefulness of salivary biomarkers in oral precancer and cancer. In a recently published study, approximately 50 mirnas were detected in saliva using reverse. In this chapter, the potential salivary biomarker signatures. Pdf role of salivary biomarkers in oral cancer researchgate. Saliva protein biomarkers and oral squamous cell carcinoma pnas. Oscar rapadogonzalez 1,2, blanca majem 3, laura muineloromay 2, ana alvarezcastro 4, anna santamaria 3, antonio gilmoreno 3,5, rafael lopezlopez 2, maria mercedes suarezcunqueiro 1. The identification of potential saliva signatures for early, noninvasive detection of oral squamous cell carcinoma oscc lead to early detection, better outcome, and survival.
Frontiers salivary biomarkers for oral squamous cell. Potential salivary mrna biomarkers for early detection of. Salivary testing, a noninvasive alternative to serum testing, can be an effective modality for diagnosis and prognosis predicting of oral cancer as well as for monitoring the patients post. Therefore, the implementation of newer screening and early detection. Pdf salivary analysis of oral cancer biomarkers researchgate. The existence of gene mutations can often be associated and used as biomarkers in order to diagnose oral cancer. Currently, oral cancer diagnosis depends on a thorough oral. Among malignancies, oral cancer is the type in which salivary examination shows the enormous benefits due to its direct interaction with the cancer cells. The identification of potential saliva signatures for early, noninvasive detection of oral. Noninvasive diagnosis of disease and monitoring of general health herenia p. Therefore, the implementation of newer screening and early detection approaches are of utmost importance which could reduce.
Oral squamous cell carcinoma accounts for more than 90% of. More accurate oral cancer screening with fewer salivary biomarkers james michael menke1, md shahidul ahsan2 and suan phaik khoo3 1a. Currently, oral cancer diagnosis depends on a thorough oral examination, usually by a dentist or other qualified health care provider, for possible signs and symptoms of the disease. Saliva is an easily accessible biofluid with immense diagnostic potential in oral cancer. We subjected our salivary oral cancer biomarkers to a phase ii validation study with an edrnbrl to assess the reproducibility of the oral cancer salivary biomarkers. Prevalidation of salivary biomarkers for oral cancer. This article aims to provide a brief overview of various salivary biomarkers and their implications in oral cancer. Longterm longitudinal studies with large populations of individuals with oral cancer and those who are at high risk of developing oral cancer are needed to validate these potential biomarkers. Salivary il8, il6 and tnf as potential diagnostic biomarkers for oral cancer haafsa arshad sahibzada 1, zohaib khurshid 2, rabia sannam khan 3, mustafa naseem 4, khalid mahmood siddique 5, maria mali 6 and muhammad sohail zafar 7,8 1 department of oral medicine, islamabad dental hospital idh, islamabad 44000, pakistan. In particular, we examine the composition and function of the saliva, with special focus on salivary biomarkers. So far, more than 100 potential oscc salivary biomarkers have been reported in the literature, based mainly on comparing. Role of salivary biomarkers in the diagnosis of oral cancer. Specific terms were used from january 1995 to march 2017 by three experts.
Salivary diagnostics, oral cancer, oral pre cancer, metabolomics. Biomarkers in the saliva have the potential to be used for screening purposes in epidemiological studies. Previous studies have revealed the clinical efficiency of salivary biomarkers for oral cancers. Salivary proteomics for oral cancer biomarker discovery. So far, more than 100 potential oscc salivary biomarkers have been reported in the literature, based mainly on comparing the levels found in oscc patients to the levels found in nonoscc normal controls see table 1 1862. Dec 10, 2019 oral cancer is the sixth most common cancer type in the world, and 90% of it is represented by oral squamous cell carcinoma oscc. Despite progress in preventive and therapeutic strategies, delay in oscc diagnosis remains one of the major causes of high morbidity and mortality. Apr 18, 2020 salivary biomarkers and their efficacies as diagnostic tools for oral squamous cell carcinoma. Pdf salivary analysis of oral cancer biomarkers iris. Salivary tools for the measurement and investigation of alterations in particular salivary molecules such as proteins, genes, enzymes, cytokines, metalloproteinases, cytokeratins, growth factors, etc. Salivary biomarkers in oral squamous cell carcinoma an. Salivary biomarkers for diagnosis of systemic diseases and. Oral cancer has emerged as an alarming public health problem with increasing incidence and mortality rates all over the world.
Prevalidation of salivary biomarkers for oral cancer detection. Review article salivary rna signatures in oral cancer detection. The aim of this study was to investigate if certain salivary biomarkers could be used for detection of common systemic diseases. Nmr 1h and c based signatures of abnormal choline metabolism in oral squamous cell carcinoma with no prominent warburg effect. Saliva becomes the prime choice for screening and identification of biomarkers because of the fallen cancer cells in the oral cavity 126. Feb 24, 2014 reported potential salivary biomarkers for oral cancer detection. Aug 30, 2019 this presentation provides an overview of the clinical applicability of salivabased biomarkers in oral cancer along with the discussion of research finding determining the clinical utility of salivary metabolites in oral leukoplakia and oral squamous cell carcinoma. Biomarkers are being used as diagnostic or monitoring tools for a wide variety of diseases, including systemic lupus erythematosus, sjogren disease, behcet disease. This presentation provides an overview of the clinical applicability of salivabased biomarkers in oral cancer along with the discussion of research finding determining the clinical utility. Over the past decade, there has been a dramatic increase in studies evaluating salivary biomarkers of stress.
Oral cancer is one of the major global public health problems and is the sixth most common human malignancy. Reported potential salivary biomarkers for oral cancer detection. Proteinbased salivary profiles as novel biomarkers for oral. Also, new pointofcare platforms such as oral uid nanosensor test are now available that will soon emerge as chairside tools. Over the past decade, there has been a dramatic increase in studies evaluating.
Sep 29, 2009 salivary testing, a noninvasive alternative to serum testing, can be an effective modality for diagnosis and prognosis predicting of oral cancer as well as for monitoring the patients post. Salivary cortisol level was used as an endocrine marker and salivary alphaamylase activity as. Biomarker, dna, mrna, oral squamous cell carcinoma, saliva decreases to as low as 1550%. To our knowledge, this was the first study illustrating the association between the salivary microbiome and pancreatic pathologies. Salivary biomarkers in oral squamous cell carcinoma an insight. In addition to traditional tissue biopsybased diagnostics, more reliable, inexpensive, and noninvasive methods are required for early diagnosis of cancer.
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