Receive the latest news, hot plasmids, discounts and more. Construction of a recombinant pires2egfparts plasmid and its. Familial alzheimers diseaseassociated presenilin 1 mutants. Acgfp1 is a green fluorescent protein gfp from aequorea coerulescens. The pires2egfp backbone also provides a puc origin of replication for propagation in e. First, we optimized the volume and concentration of the transgenic construct, linearized pires2egfp plasmid, by in vitro transfection of. Pdf construction of an egfp expression plasmid under. Please use this form to recommend updates to the information in zfin. Does pires2 express more egfp than the mrna before the ires. Restriction map and multiple cloning site mcs of pires2egfp vector. Learn about the latest plasmid technologies and research tools. Genes inserted into the mcs should include an initiating atg codon. Exciting gfp intensely for extended periods may generate free radicals that are.
Egfp is listed in the worlds largest and most authoritative dictionary database of abbreviations and acronyms the free dictionary. Acgfp1 is a green fluorescent protein gfp from aequorea. Welcome to vector database vector database is a digital collection of vector backbones assembled from publications and commercially available sources. The clone can be used alone simply as a marker or in conjunction with a gene insert. We appreciate as much detail as possible and references as appropriate. This page is informational only this vector is not available from addgene. The pires2 zsgreen1 backbone also provides a puc origin of replication for propagation in e. Ascl 2,475 fsel 2,458 bamhl 1,712 poo sv40 poiya site. Construction of an egfp expression plasmid under control of t7 promoter and ires sequence for assay of t7 rna polymerase activity in mammalian cell linesconstruction. In vitro gene transfer and transgene expression in testis. This is a free resource for the scientific community that is compiled by addgene. Clontech takara download free trial get snapgene viewer. Have questions about your order, deposit, or a plasmid.
Efficient multicistronic expression of a transgene in human. Irescontaining bicistronic vector for expressing a gene together with egfp. This is a free resource for the scientific community that is compiled by addgene this page is informational only this vector is not available from addgene please contact the manufacturer for further details. Egfp fragment from pegfpn1 and the smaibstxi ires frag ment from pires2egfp into the ecorismai site of pdsred. Looking for online definition of egfp or what egfp stands for. Subsequently, the recombinant pires2egfparts plasmid was transfected into lx2 cells by fugene 6 transfection reagent, and the. Hiv1 cppt hiv1 rre efs promoter dyk p2a plenticas9egfp. Construction and identification of pires2 ngfvegf 165. The vector is designed to allow efficient flow cytometric detection of transiently transfected. Intratesticular injection followed by electroporation. Ascl 2,475 fsel 2,458 bamhl 1,712 poo sv40 poiya site 3400 pbr322 origin pcs2egfpfa ampicillin 4,839 bp potts lab simian cmv ie94 sp6 promoter.
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